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. 2022 Feb 17;23:144. doi: 10.1186/s12864-022-08312-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Distribution and characteristics of differentially methylated cytosines (DMCs), or differentially methylated regions (DMRs). a & d Total numbers of all hyper- (red) or hypomethylated (cyan) DMCs (q-value ≤ .05; panel a) or DMRs (with a minimum absolute methylation difference of 10% between CONTROL and COLD; panel d). b & e Fraction of hypermethylated (red) or hypomethylated (cyan) DMCs (b), or DMRs (e) in CpG, CHG or CHH context overlapping an upstream region (2.5 kb upstream of TSS), 5’UTR, CDS, intron or 3’UTR. Percentages (x- axis) refer to the fraction of associated DMCs (or DMRs) compared to all DMCs (or DMRs) of the same context and the same direction of change in methylation, respectively. Numbers indicate the count of differentially methylated positions or regions constituting the corresponding bar. c & f Distribution of differential methylation at single positions (c) or extending over multiple cytosines within the same sequence context in larger DNA stretches (f) over the chromosomes of Beta vulgaris. Position along the y-axis indicates the difference in methylation between CONTROL and COLD