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. 2022 Feb 17;23:144. doi: 10.1186/s12864-022-08312-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Methylation profiles along lowly or highly expressed genes. Profile plots depict data corresponding to methylation and expression in one (representative) sample of CONTROL (profiles on the left), or of COLD (profiles on the right). Genes were ranked and assigned to one of six categories (color-coded) based on their expression level (FPKM; i.e. after normalizing for gene lengths) in a given sample. All genes were split into 30 windows (TSS to TTS, including introns) and window-averages of all genes within the same category (i.e. with similar expression levels) are plotted as an individual line profile, overall depicting the distribution of methylation for groups of lowly, intermediate (orange to green) or highly expressed genes. Gene flanks extend another 15 windows (half the gene length) 5′ of the TSS (upstream) or 3′ of the TTS (downstream), respectively. The inset in the lower panel (between CHH profiles of CONTROL and COLD) compares peak levels of mCHH (5′ region of genes), to illustrate decreased mCHH levels of the COLD sample throughout different expression categories