FIGURE 1.

Adaptation to 6‐day CL treatment induces weight loss and increased WAT mitochondrial proteins. Mice were treated with I.P. injections of CL (1 mg/kg) for 6 days. (a, b) Body weight and food intake measured daily throughout treatment (n = 6–7 mice/group). Twenty‐four hours following the last CL treatment adipose tissue depots were dissected, weighed (c; n = 6–7 mice/group), and analyzed for protein content via western Blot (d, e; n = 5–6 mice/group). Proteins of interest were expressed relative to an internal ponceau loading control from the same respective gel as the protein of interest. Please note that a ponceau stain was used for correction purposes for each protein of interest but for space purposes only one representative ponceau is shown for each tissue. Differences between groups were analyzed by two‐tailed unpaired t‐test. *p < 0.05 between indicated groups (vehicle vs chronic CL). All data are presented as mean ± SEM