FIGURE 3.

The acute metabolic effects of CL dissipate with repeated use in high fat diet‐fed animals. All mice in these sets of experiments were fed a 45% kcal high‐fat diet for 4 weeks prior to drug treatment then adapted to CL or saline injections for 6 days. On the seventh day mice were treated acutely with CL or saline. (a, b) Body weight and food intake were measured daily throughout treatment (n = 12–13 mice/group). (c; n = 6 mice/group) Four hours following the acute CL treatment adipose tissue depots were dissected and weighed. (d; n = 6–7 mice/group) Blood glucose was measured each hour for 4 h for determination of AUC (glucose curve inset). Four hours following acute treatment serum and WAT depots were collected for analysis of NEFA (e; n = 6–7 mice/group), glycerol (f; n = 6–7 mice/group), and insulin (g; n = 4–6 mice/group). Differences between groups were analyzed by two‐way ANOVA. *p < 0.05 between indicated groups. All data are presented as mean ± SEM