. 2022 Feb 18;10(4):e15187. doi: 10.14814/phy2.15187

TABLE 1.

White adipose tissue explant metabolites

	Treatment groups				p Value
	Control‐vehicle	Control‐CL	Adapted‐vehicle	Adapted‐CL	Acute CL	Chronic	Acute × chronic
iWAT NEFA (mM/g tissue)	0.014 ± 0.0015^a	0.027 ± 0.0034^b	0.013 ± 0.0005^a	0.015 ± 0.0010^a	0.0015	0.0042	0.0124
iWAT GLYCEROL (mM/g tissue)	0.024 ± 0.0018	0.040 ± 0.0045	0.019 ± 0.0011	0.030 ± 0.0008	<0.0001	0.0111	0.2595
eWAT NEFA (mM/g tissue)	0.012 ± 0.0009^a	0.037 ± 0.0017^b	0.013 ± 0.0011^a	0.021 ± 0.0016^c	<0.0001	<0.0001	<0.0001
eWAT GLYCEROL (mM/g tissue)	0.022 ± 0.0014^a	0.050 ± 0.0030^b	0.022 ± 0.0021^a	0.038 ± 0.0025^c	<0.0001	0.0179	0.0199

Mice were adapted to CL with daily I.P. injection for 6 days in vivo before animals were anesthetized, WAT was dissected, and treated acutely in vitro. Data are represented in mM per hour, per gram of adipose as the mean ± SEM. eWAT, epididymal white adipose tissue; iWAT, inguinal white adipose tissue; NEFA, nonesterified fatty acids; Acute CL, p value for main effect of acute CL 316,243 treatment; Chronic, p value for main effect of adaptation to 6 days of CL 316,243 treatment; acute × chronic, p value for interaction between acute CL treatment and adaptation to chronic CL treatment. Different superscript lowercase letters denote a significant post hoc comparison (n = 5 per group).