Fig. 7.
c-fos mRNA and MEK-1. The increase in c-fos mRNA caused by addition of 100 nM (FAFN)BB6–14 to NCI-H1299#5 cells was inhibited by 10 μM (d-Arg1, d-Pro2, d-Trp7,9, Leu11)substance P ((APTTL)SP) and 10 μM PD98059. Also, if NCI-H1299#5 cells were transfected with a dominant-negative (DN) MEK-1, 100 nM (FAFN)BB6–14 had little effect on c-fos mRNA. (Bottom) Ethidium bromide staining. This experiment is representative of two others.