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. 2022 Feb 8;7(3):e145475. doi: 10.1172/jci.insight.145475

Figure 1. Ito,f-related proteins were downregulated in CKD rats with a high levels of IS.

Figure 1

(A) Flow chart of animal experiments. (B and D) Determinations of IS levels in serum (B) and heart tissues (D) at 8 weeks after right nephrectomy (n = 5 per group). (C and E) Measurements of IS levels in serum (C) and heart tissues (E) after 8 weeks of IS treatment (n = 6 per group). (F and I) Representative immunoblots (F) and average data (I) of Kv4.2, Kv4.3, and KChIP2 proteins in vehicle and IS treatment groups (n = 6 per group). (G and H) Representative immunoblots (G) and average data (H) of Kv4.2, Kv4.3, and KChIP2 proteins in sham, CKD, and CKD plus BB536 groups (n = 5 per group). (J) Representative IHC images of Kv4.2 and Kv4.3 proteins in rats. Scale bar: 100 μm. Data are presented as mean ± SEM. Statistical analysis was performed using 2-tailed Student’s t test (C, E, and I) and 1-way ANOVA followed by Bonferroni post hoc test (B, D, and H). *P < 0.05, **P < 0.01.