FIG. 1.

Amplification and detection of parvovirus B19-specific DNA by using LC-FRET technology. Primers were chosen from the gene encoding NS-1. For primer sequences (NS-1a and NS-1a′) and locations, see Table 1. (A) Detection of the B19-specific amplicon by use of a hybridization probe-and-anchor pair. FL, 3′ fluorescein dye label of the probe; LC₆₄₀, 5′ LC-Red 640 dye label of the anchor. (B) IC amplicon constructed for coamplification with the specific fragment. S and B, unique SphI and a BglII sites, respectively, introduced by site-directed mutagenesis. The original anchor sequence between these restriction sites was replaced with a semisynthetic IC sequence (white bar) which can be detected using the IC anchor labeled with LC-Red 705.