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. 2022 Feb 8;7(3):e149636. doi: 10.1172/jci.insight.149636

Figure 3. 4PBA treatment improves translocation and osteoblastic transition of hypertrophic chondrocyte in G610C OI mice.

Figure 3

(A) Effect of overnight treatment with 4PBA (5 mM, filled square dots) on Col10a1, Alp, and Bsp mRNA in G610C chondrocyte pellets cultured for 3 weeks relative to the mean values in the control group treated with PBS (circle dots, n = 5 per group, 2-tailed Student’s t test). (B) Representative images of the trabecular area of tibiae from 3-week-old Col10a1-Cre;Ai9;Col1a1 2.3-GFP and Col10a1-Cre;Ai9;Col1a1 2.3-GFP;G610C mice treated with either PBS or 4PBA (0.4 mg per day) for 10 days. Scale bar: 100 μm. (C) The number of HC-derived cells (red + yellow cells) per 0.01 mm2 in the trabecular area (n = 4 per group; 2-way ANOVA; treatment-genotype interaction, P < 0.05). (D) The percentage of osteoblasts (yellow cells) in HC-derived cells (red + yellow cells) in the trabecular area (n = 4 per group; 2-way ANOVA; treatment-genotype interaction, P < 0.05). Data are shown as mean ± SEM.