Figure 2. Reduced natriuresis to the ENaC blocker amiloride in Epac1^–/–, Epac2^–/–, and Epac1&2^–/– mice.

Summary graphs comparing urinary Na⁺ levels (A) and K⁺ levels (B) in Epac WT, Epac1^–/–, Epac2^–/–, and Epac1&2^–/– mice kept on Na⁺-deficient diet for 7 days at the baseline and following i.p. injection of amiloride (2 mg/kgBW). Measurements from the same animal are connected with lines. In both cases, urine samples were collected over a 6-hour period. Relative reductions (in %) in urinary K⁺ levels are shown for each tested group. *P < 0.05 versus Epac WT mice + Amiloride; 1-way ANOVA with post hoc Tukey test was used.