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. Author manuscript; available in PMC: 2022 Feb 18.
Published in final edited form as: Anal Chem. 2021 Nov 17;93(47):15728–15735. doi: 10.1021/acs.analchem.1c03652

Figure 2.

Figure 2.

Precursors of Protein G and total ETD product ion signal are evaluated as a function of ETD reaction time. The reactions of Protein G’s [M + 28H]+28 at 767 m/z with (a) standard ETD and (b) with ETD-PIP are compared side-by-side. ETD-PIP is also demonstrated on Protein G’s (c) [M + 26H]+26 at 825 m/z, (d) [M + 24H]+24 at 894 m/z, (e) [M + 22H]+22 at 976 m/z, and (f) [M + 20H]+20 at 1073 m/z.