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. 2022 Feb 8;7(3):e156236. doi: 10.1172/jci.insight.156236

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© 2022 Liao et al.

This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative FACS plots of myocardial cells. P1, macrophages; P2, neutrophils; Q1, Tim4– infiltrating macrophages; Q2, Tim4+ cardiac resident macrophages. (B) Changes in cardiac cells after ISO treatment. The percentage of cardiac macrophages was calculated against all cells counted by flow cytometry, excluding debris. n = 14–16. (C) The percentages of Tim4⁺ and Tim4^– cardiac macrophage subsets (total cardiac macrophages, 100%). n = 9–10. (D) Representative FACS plots of peripheral blood monocytes. P1, monocytes (CD11b⁺CD115^hi); Q1, Ly6C^lo monocytes; Q2, Ly6C^hi monocytes. (E) Total monocytes in the blood at different days after ISO treatment (all white cells, 100%). n = 8–11. (F) The percentages of Ly6C^hi and Ly6C^lo monocytes (monocytes, 100%). n = 8–10. Dosing: ISO: 200 mg/kg. P values were from 1-way ANOVA (B and E) and 2-way ANOVA (C and F) post hoc test with Tukey correction. d, days after ISO injection.