Figure 3.

CircUHRF1 functions as a ceRNA of miR-1306-5p. ENCORI database was used to predict putative downstream miRNA targets for circUHRF1. (http://starbase.sysu.edu.cn, CLIP data: high stringency (≥3)). a: RNA immunoprecipitation (RIP) and RT-qPCR assays were conducted to analyze the binding capability of circUHRF1 to AGO2 protein in PANC1 (a) and AsPC-1 (b) cells. B: RNA pull-down assay using Bio-NC probes and Bio-circUHRF1 probes was performed to detect putative miRNA levels in PANC1 cells. c: The predicted binding sites between circUHRF1 and miR-1306-5p were shown. d: Relative luciferase activity measurement in HEK-293 T cells and PANC1 cells transfected with miR-1306-5p mimic and reporter vectors harboring WT or Mut circUHRF1sequences. e: Relative expression of miR-1306-5p in circUHRF1 knockdown PANC1 and AsPC-1 cells was measured by qRT-PCR. f: Relative expression of miR-1306-5p in adjacent normal tissues (n = 12) and PDAC tissues (n = 26) was detected by qRT-PCR. g: The expression correlation between circUHRF1 and miR-1306-5p in PDAC tissues was statistically calculated using spearman analysis. All experiments were performed three times, and data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.