Figure 4.

MiR-1306-5p inhibits PDAC cellular progression and reverses the EMT process. a: MiR-1306-5p overexpression cell models were constructed by stably transfecting miR-1306-5p mimics and its normal control mimics into PANC1 and AsPC-1 cells. Transfection results were detected by qRT-PCR. B-D: CCK-8 (b), EdU (c), and Colony formation assays (d) were performed to evaluate cell proliferation abilities. e: Apoptotic rates of transfected cells were evaluated by Flow cytometry assay. f: Transwell assay was applied to measure cell migration levels. F: Western blot was conducted to detect the EMT-related proteins in indicated groups. All experiments were performed three times, and data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.