Figure 6.

CircUHRF1 knockdown decreases ARL4C expression to inhibit PDAC progression via sponging miR-1306-5p. a: PANC1 and CFPAC1 cells were stably transfected with Si-NC, Si-circ#1, Si-circ#1 + OE-NC, Si-circ#1 + OE-ARL4C as indicated, transfection efficiencies were detected by RT-qPCR. B-G: CCK-8 (b-c), EdU (d-e), and Colony formation assays (f-g) were performed to evaluate cell proliferation abilities. h-i: Cell apoptotic rate was evaluated by Flow cytometry assay. j-k: Transwell assay was applied to measure cell migration levels. j: Western blot was conducted to detect the EMT-related protein levels in indicated groups. All experiments were performed three times, and data were presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.