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. 2021 Dec 17;21(3):247–260. doi: 10.1080/15384101.2021.2015671

Figure 2.

Figure 2.

CircGOT1 generated from exon 8 of GOT1 via back-splicing. (a). Profile from the UCSC (https://genome.mdc-berlin.de/index.html) illustrated that circGOT1 generated from its host gene GOT1. (b-c). The qPCR analysis was used to determine the expression of circGOT1 and GOT1 after treatment with 2 mg/mL actinomycin D for 4 h, 8 h, 12 h. (d-e). The qPCR analysis was used to validate the expression of circGOT1 and GOT1 after treatment with 3 U/µg RNase R. (f-g). The qPCR analysis was used to validate the expression of circGOT1, U6, and GAPDH in nucleus and cytoplasm, respectively. ***P < 0.001.