Figure 3.

mbsAb-PD-L1×4-1BB does not induce infiltration of CD8⁺ T cells in the liver, increase liver wet weight or liver enzyme activity in peripheral blood in healthy C57BL/6 mice.

C57BL/6 mice received three IP injections with mbsAb-PD-L1×4-1BB or the indicated control antibodies (250 µg each) on days 0, 3, and 6. (a) Livers were resected on day 16, and sections were stained with H&E (left panels) or using CD8 antibodies (right panels). Representative images are shown. Arrowheads indicate lymphocytic infiltrates. (b) Quantification of lymphocytes in the liver sections (n = 5) (c) Quantification of CD8⁺ cells in the liver sections. (d) Wet weights of livers 16 days after treatment (n = 5). (e) ALT enzyme activity was measured in sera collected 16 days after the first treatment before the mice were sacrificed. Data from individual mice are shown, as well as group mean ± SD (n = 10). Statistical significance was determined by Ordinary one-way ANOVA with Dunnetts’s multiple comparisons test (treatment groups vs. isotype control). *P < .05; **, P < .01; ***, P < .001; ****, P < .0001.