Figure 2.

NCAPH promotes the cell proliferation of BC cells. (a,b) The mRNA and protein levels of NCAPH in SW780 cells transfected with NCAPH expression vector and control vector were determined by qRT‐PCR and Western blot. (c,d) The mRNA and protein levels of NCAPH in UMUC3 cells transfected with NCAPH siRNA1-3 and negative control (siNC) were determined by qRT‐PCR and Western blot. (e-h) The effect of NCAPH on cell proliferation of BC cells was determined by BrdU assay (Scale bar, 100 μm) and MTT assay. The data were presented as the mean ± SD. **P < 0.01. In g and h, no significant difference between Vector and NCAPH, siNC and siNCAPH-2/3 group at 0 and 24 h.