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. 2022 Feb 4;13:826771. doi: 10.3389/fphar.2022.826771

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Copyright © 2022 Xiong, Li, Mei, Ding, Shen, Zhu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Efficiency of NPs taken up by cell lines and the mouse cervix. (A) Representative images of green fluorescent protein expression in SiHa, HeLa, S12, CaSki, MS751, C33A, and HEK293 cells after 72 h of nanoparticle (PBAE537/GFP 60:1) treatment. (B) Flow cytometry was used to determine the efficiency of nanoparticles transfected with PBAE537/GFP at different weight ratios (20:1, 40:1, 60:1, and, 80:1) and HP/GFP (1:1) for the expression of the green fluorescent protein. bPEI is a cationic polymer with transfection ability used as a control. (C) Representative images of red fluorescent protein expression in the cervix of the C57BL/6 mice treated with nanoparticles composed of different weight ratios of PBAE537 to RFP (10 μg RFP, once daily, for 3 days) compared with the control cervix treated with sodium acetate alone without nanoparticles. Scale, 20 μm.