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. Author manuscript; available in PMC: 2022 Feb 18.
Published in final edited form as: Clin Exp Metastasis. 2017 Feb 17;34(2):185–196. doi: 10.1007/s10585-017-9839-9

Figure 4. Functions of astrocytic factors on Reelin transcription.

Figure 4.

(A) Real-time PCR quantification of Reelin expression in Her2+ (BBM1) cells co-cultured with astrocytes (Astro cc) in the presence of 5-azacytidine for 24 h in serum-free media, followed by an additional 48 h of growth with or without 5-azacytidine (n = 3, **p<0.05, ***p<0.01, RQ = relative quantification compared to Actin). Bars indicate SEM. (B) Reelin promoter region showing cis-elements for transcription factors. (C) Set1 histone methyltransferase and GATA1 transcription factor were knocked down in BBM1 cells by shRNA and cocultured with astrocytes for 24 h. Real-time PCR quantification of Reelin expression (n = 3, ***p<0.01, bars indicate SEM).