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. 2022 Feb 15;44(1):293–303. doi: 10.1080/0886022X.2022.2039194

Figure 1.

Figure 1.

miR-122-5p is induced in renal tubules in DN mice. Eight-week-old C57BL/6J mice were injected with 50 mg/kg STZ for 5 consecutive days. Control mice were injected with normal saline. The mice were euthanized after 12 weeks. (A) body weight changes in control and STZ mice; (B) Blood glucose concentrations in each group; (C) urinary NAG levels; (D) urinary ACR levels; (E) representative images of hematoxylin–eosin (HE) staining, periodic acid-Schiff (PAS) and Masson staining, scale bar: 50 μm; (F) in situ hybridization showing miR-122-5p increase in kidney tissues after STZ treatment. Representative images of double immunostaining with miR-122-5p and proximal tubule marker, Lotus tetragonolobus lectin (LTL). Scale bar: 50 μm; (G) qPCR analysis of miR-122-5p in mouse kidneys with STZ treatment or normal saline. The level of miR-122-5p was normalized to the level of U6 (internal loading control) of the same samples to determine the ratio with the ratio of control mice arbitrarily set as 1.All the values are expressed as mean ± SD (n = 6), *p < 0.05.