Skip to main content
. 2022 Feb 15;44(1):293–303. doi: 10.1080/0886022X.2022.2039194

Figure 5.

Figure 5.

miR-122-5p attenuates high glucose-induced tubular injury through targeting FIH-1. BUMPT cells were transfected with pHIF-1α plasmid, FIH-1 plasmid, or negative control (NC) for 24 h and then treated with high glucose (35 mM) for another 24 h. Control cells were maintained in normal medium. (A) Immunoblot of HIF-1α to confirm the successful delivery of pHIF-1α plasmid; (B) quantification analysis of the related band intensity. Values are expressed as Mean ± SD (n = 4), *p < 0.05. (C) Immunoblot of cleaved-caspase 3 from BUMPT cells with negative control (NC) or HIF-1α plasmid transfection. GAPDH was used as loading controls. (D) Quantification analysis of the related band intensity. Values are expressed as mean ± SD (n = 4), *p < 0.05. (E) Representative images of TUNEL staining, scale bar: 100 μm; (F) immunoblot of cleaved-caspase 3, GAPDH was used as loading controls. (G) Quantification analysis of the related band intensity. Values are expressed as mean ± SD (n = 4), *p < 0.05.