Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 4;9:804210. doi: 10.3389/fnut.2022.804210

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Jhong, Tsai, Yang, Chou, Lee, Yeh, Huang and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Heat-killed probiotics reduced IL-6 and NO levels through LPS stimulation in mouse RAW 264.7 macrophages. An appropriate number of cells were seeded in a 24-well plate. Probiotics (L. reuteri, L. rhamnosus, L. salivarius, and five strains of L. paracasei) were cultured in MRS broth for 20 h, with the bacterial concentration adjusted through centrifugation. These bacteria were heat killed (90°C for 30 min) and then added to cells with or without LPS stimulation. After 20 h, the culture supernatant was collected for measurement of the IL-6 production (A,B) and NO (C). *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the group treated with LPS alone.