CD1D-deficient mice have similar levels of circulating total IgM and lower levels of circulating total IgG compared to WT controls prior to transfusion. Serum was collected from unimmunized 8-week-old WT and CD1D-KO mice. (A) Total IgM and (B) total IgG were measured by ELISA. Each dot represents one mouse. Bars on scatter plots represent median values. Figure shows a representative experiment out of 4. *p < .05, **p < .01, ***p < .001, ****p < .0001, ns p > .5. IgG, immunoglobulin G; IgM, immunoglobulin M; ELISA, enzyme-linked immunosorbent assay; WT, wild type