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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Transfusion. 2021 Jun 28;61(7):2169–2178. doi: 10.1111/trf.16554

FIGURE 4.

FIGURE 4

CD1D-deficient mice generate similar anti-RBC IgM alloantibody levels compared with WT mice in response to transfused stored RBCs. WT and CD1D-KO mice were transfused with stored HOD RBCs. Serum was collected 7 dpt and the levels of anti-HOD alloantibody generation were measured by (A) flow cross-match presented as adjusted MFI and (B) limiting dilution ELISA presented as IgM titer. Each dot represents one mouse. Bars on scatter plots represent median values. Figure shows a representative experiment out of 4. *p < .05, **p < .01, ***p < .001, ****p < .0001, ns p > .5. dpt, days post-transfusion; IgM, immunoglobulin M; ELISA, enzyme-linked immunosorbent assay; MFI, mean fluorescence intensity; WT, wild type