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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Transfusion. 2021 Jun 28;61(7):2169–2178. doi: 10.1111/trf.16554

FIGURE 7.

FIGURE 7

CD1D-deficient mice generate similar anti-RBC alloantibody levels compared with WT mice in response to transfused fresh RBCs. WT and CD1D-KO mice were transfused with fresh HOD RBCs. Serum was collected 7 and 14 dpt. (A) The levels of anti-HEL IgM and IgG generation were measured by limiting dilution ELISA and presented as IgM or IgG titer. (B) The levels of IgG subtypes were measured by single dilution ELISA at 1:100 dilution and presented as OD. Each dot represents one mouse. Bars on scatter plots represent median values. Figure shows a representative experiment out of 2. *p < .05, **p < .01, ***p < .001, ****p < .0001, ns p > .5. dpt, days post-transfusion; IgG, immunoglobulin G; IgM, immunoglobulin M; ELISA, enzyme-linked immunosorbent assay; OD, optical density; WT, wild type