Fig 3. Morphological features of cells delineating developing biliary lumen.
(A) Tight junctions are detected between HNF4- cholangiocytes and between cholangiocytes and HNF4+ hepatoblasts; but not between adjacent hepatoblasts. (B) Proliferating SOX9+ cholangiocytes and HNF4+ hepatoblasts are detected by Ki67-staining in E16.5 embryonic livers. E-cad: E-cadherin. (C) Apical constriction in cholangiocytes. Developing ducts are delineated by white dotted lines. White size bar, 20 micrometer; yellow size bar 10 micrometer. PV: portal vein.