Fig 1. V-59 inhibits Mtb growth in an Rv1625c-dependent mechanism.

(A and B) Impact of V-59 on Mtb growth in cholesterol media (A) and in media containing cholesterol and acetate (B). V-59 (10 μM) was added to the cultures every three days, and DMSO is the vehicle control. Data are from two experiments, with three technical replicates each (*P < 0.05, One-way ANOVA with Sidak’s multiple comparisons test). (C) Effect of V-59 on growth of Mtb in murine macrophages. Macrophages were infected at an MOI of 2 and treated with V-59 (25 μM) or DMSO. Data are from three experiments with two or more technical replicates each (****P < 0.0001, One-way ANOVA with Sidak’s multiple comparisons test on fold-change in CFU’s normalized to DMSO). All data are means ± SD.