Fig 6. V-59 treatment and induction of TetOn-cAMP are associated with shared transcriptional changes to cholesterol utilization genes.

RNA-seq analysis quantifying differentially expressed genes from Mtb grown in cholesterol media, following V-59 treatment, or induction of TetOn-cAMP with Atc. Genes depicted are in the KstR regulons and involved in cholesterol utilization. MCC = methylcitrate cycle. Data are displayed as log₂ fold change in gene expression in response to cAMP-inducing vs. control treatment (“cAMP” = Tet-On cAMP Atc vs. EtOH, “WT” = WT V-59 vs. DMSO, “∆” = ΔRv1625 V-59 vs. DMSO, “C” = Comp_Full V-59 vs. DMSO). Also shown are differentially expressed genes intrinsic to ∆Rv1625 (“Δ/WT” = ∆Rv1625 DMSO vs. WT DMSO). Data are from two technical replicate samples from one experiment (*adjusted P-value ≤ 0.05).