. 2021 Jul 21;143(30):11510–11519. doi: 10.1021/jacs.1c03939

Table 1. Kinetic Parameters Associated with the Aptananozymes I–V and Control Systems^a.

aptananozyme	V_max (μM min^–1)	K_M (μM)	k_cat (10^–3 s^–1)	k_cat/K_M (s^–1 M^–1)
IV	2.01 ± 0.1	181 ± 32	2.02 ± 0.1	11.2
III	1.97 ± 0.08	230 ± 31	1.98 ± 0.08	8.6
I	1.71 ± 0.04	232 ± 17	1.72 ± 0.04	7.4
II	1.52 ± 0.1	252 ± 57	1.53 ± 0.1	6.0
V	1.28 ± 0.05	324 ± 43	1.29 ± 0.05	4.0
scrambled	0.73 ± 0.04	464 ± 70	0.74 ± 0.04	1.6
separated^b	0.04		0.04

All experiments were performed in a 5 mM MES buffer solution, pH 5.5, that included 5 mM MgCl₂, 100 mM NaCl, and 0.2 μg·mL^–1 of the respective aptananozymes or control system and 5 mM H₂O₂.

The separated Cu²⁺ ion-functionalized C-dots/DBA system shows pseudo-first-order kinetics: k = 0.04 × 10^–3 s^–1.

Table 1. Kinetic Parameters Associated with the Aptananozymes I–V and Control Systemsa.

Table 1. Kinetic Parameters Associated with the Aptananozymes I–V and Control Systems^a.