Table 1:
Reagents and Chemicals.
| Name | Company | Cat # |
|---|---|---|
| DMEM medium | Mediatech Inc (Manassas, VA) | 10-014-CM |
| F-12 medium | HyClone (Logan, UT) | SH30026.01 |
| Antibiotics and Trypsin | HyClone | |
| FBS | Corning (Corning, NY) | MT35010CV |
| Glucose | Sigma (St. Louis, MO) | G8270 |
| Azaserine | Sigma | A-1164 |
| Hydrogen peroxide | Sigma | 95321 |
| Mounting medium | Molecular Probes (Eugene, OR) | P36935 FeSO4, Iron (II) sulfate heptahydrate) Sigma 7782-63-0 |
| Ferrostatin 1 | Sigma | SML0583 |
| Deferasirox (DFX) | Sigma | SML2673 |
| Zileuton | Sigma | Z4277 |
| Selenium | Sigma | 229865 |
| Primary Antibodies | Dilution | Species | Company | Cat # (WB) |
|---|---|---|---|---|
| TXNIP | 1:1000 | Rabbit | Cell Signaling (Danvers, MA) | 14714S |
| SOD1 | 1:1000 | Rabbit | Proteintech (Tucson, AR) | 10269-1-AP |
| Trx1 | 1:500 | Rabbit | Santa Cruz | SC-20146 |
| ZO-1 | 1:1000 | Rabbit | Cell Signaling | #13663 |
| Tubulin | 1:1000 | Rabbit | Cell Signaling | 2144S |
| Aco1 | 1:1000 | Mouse | Proteintech | 12406-1- AP |
| NCOA4 | 1:1000 | Rabbit | Cell Signaling | 66849S0 |
| Ferritin L | 1:3000 | Rabbit | Proteintech | 10727-1-AP |
| LC3B | 1:3000 | Rabbit | Pierce (Rockville, IL) | L10382 |
| Secondary Antibodies | Dilution | C Company | Cat # |
|---|---|---|---|
| Anti-mouse 2°ry antibody HRP | 1:5000 (WB) | S Sigma | A5278 |
| Anti-rabbit 2°ry antibody HRP | 1:5000 (WB) | S Sigma | A6154 |
| Anti-goat 2°ry antibody HRP | 1:5000 (WB) | S Santa Cruz | sc-2020 |
| IF: | |||||
|---|---|---|---|---|---|
| ZO-1 Primary Ab | 1:200 | (IF) | Rabbit | Cell Signaling | #13663 |
| Anti-rabbit 2°ry Ab | 1:400 | (IF) | Goat | Jackson | 111-585-176 |
| Alexa Fluor 594 | (West Grove, PA) |