Table I.
Characteristics of the genes investigated in this study.
| Gene Name | Description/function | Molecular Weight of pDNA vector (kDa) | Molecular Weight of IVT-mRNA (kDa) | Molecular weight of ORFa pDNA (kDa) | Molecular weight of ORFa mRNA (kDa) |
|---|---|---|---|---|---|
| EGFP | MonoCis/fluorescent marker | 3201 | 401 | 444 | 231 |
| IL13 | MonoCis/control for small size gene | 2979 | 286 | 271 | 141 |
| mCherry | MonoCis/fluorescent marker/control for medium size gene | 3165 | 382 | 439 | 228 |
| KLF4 | MonoCis/control for large size gene | 3590 | 603 | 873 | 453 |
| IL13-2A-EGFP | BiCis/co-expression of a gene with marker | 3467 | 539 | 760 | 394 |
| mCherry-2A-EGFP | BiCis/co-expression of a gene with marker | 3634 | 625 | 927 | 481 |
| KLF4-2A-EGFP | BiCis/co-expression of a gene with marker | 4077 | 855 | 1370 | 711 |
aORF open reading frame.