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. 2021 Jul 14;29(1-2):81–93. doi: 10.1038/s41434-021-00258-6

Fig. 3. Correction of rd9 retinal pathology in germline treated mice.

Fig. 3

Confocal images of retina from control C57BL/6 (Bl6) (n = 2), naïve rd9 (n > 3), and CRISPR-treated (rd9*) mice (n > 3). A, B Cone opsins (M-opsn, S-Opsn) are mislocalized throughout cone photoreceptors in young rd9 retina but correctly localized to the outer segments (OS) in both Bl6 and rd9* retina. C Rhodopsin (Rho1D4) is also mislocalized throughout rod photoreceptors in older (≈8 months) rd9 retina but expressed normally in Bl6 and rd9* retina. D Activated Muller glia, as detected by GFAP, are identified in rd9 retinas but in Bl6 and rd9* tissue, expression is restricted to the outer plexiform (OPL) and nerve fiber layers (NFL). DAPI was stained in all images but only shown on the left of each panel. All scale bars indicate 50 μm. Inner segments (IS), outer nuclear layer (ONL), inner nuclear layer (INL), inner plexiform layer (IPL), and ganglion cell layer (GCL) also labeled for reference. All rd9 and rd9* retinas were processed and imaged blind.