Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 14;29(1-2):81–93. doi: 10.1038/s41434-021-00258-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — A Confocal images of representative AAV-treated rd9 retinal cross sections demonstrating rescue. Expression of RPGR-ORF15 is observed only in ORF15-targeted (AAV CRISPR-ORF15) but not non-targeted (AAV CRISPR-NT) retina, as indicated by a subset of cells positive for RPGR-570 signal at the connecting cilium. The ciliary rootlet is stained with Rootletin (Rtltn). Right panels show desaturated 570 channel to better visualize positive signal. B Lower magnification image of staining depicted in panel (A) to better show broad distribution of correction in treated retina. Lower panel shows desaturated 570 channel. Scale bars indicate 20 μm. Injections were performed in 3–6 week old rd9 mice, and tissues were collected and analyzed 8–12 weeks post injection.