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. 2021 Dec 22;41(8):1178–1189. doi: 10.1038/s41388-021-02084-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Western blot (left panel) and densitometry quantification (right panel) showing the protein expression of MYC target genes upon the overexpression of miR-138 (138) in HCT116 and DLD-1 cell lines. B RT-qPCR analysis of CDK4, CDK6 and p27 transcript enrichment upon biotinylated miR-138 pulldown in HCT116 and DLD-1 cell lines. C The sequences of the wild-type (WT) and mutant miR-138 MREs on the CDK6 transcript (top panel) and the effect of miR-138 overexpression on the luciferase activity of the MRE-reporter constructs (bottom panel) in HCT116 (left panel) and DLD-1 (right panel) cell lines. RC denotes the reverse complement of miR-138 which was used as positive control. D Western blot (top panel) and densitometry quantification (bottom panel) of p27 protein expression upon the knockdown of MYC in HCT116 and DLD-1 cell lines. Mean ± SEM; N ≥ 3. *P < 0.05; **P < 0.01; ***P < 0.001.