KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| chicken anti-GFP | Aves | GFP-1020, RRID:AB_10000240 |
| goat anti-ChAT | Millipore | AB144p, RRID:AB_2079751 |
| rabbit anti-GFP | Invitrogen | A11122, RRID:AB_221569 |
| rabbit anti-phox2b | Jean-François Brunet lab | N/A |
| mouse anti-phox2b | Santa Cruz Biotechnology | B-11, sc-376997, RRID:AB_2813765 |
| rat anti-RFP | Chromotek | 5F8, RRID:AB_2336064 |
| donkey anti-chicken 488 | Jackson ImmunoResearch | 703-545-155, RRID:AB_2340375 |
| donkey anti-chicken Cy5 | Jackson ImmunoResearch | 703-175-155, RRID:AB_2340365 |
| donkey anti-goat Cy5 | Jackson ImmunoResearch | 705-606-147, RRID:AB_2340438 |
| donkey anti-rabbit 488 | Jackson ImmunoResearch | 711-545-152, RRID:AB_2313584 |
| donkey anti-rabbit Cy5 | Jackson ImmunoResearch | 712-165-153, RRID:AB_10895300 |
| donkey anti-rat Cy3 | Jackson ImmunoResearch | 711-495-152, RRID:AB_92645 |
| goat anti-mouse Alexa Fluor 647 | Invitrogen | 21236, RRID:AB_2535805 |
| Bacterial and virus strains | ||
| oG SiR G-Deleted Rabies-FlpO-mCherry | The Viral Vector Core at the Salk Institute for Biological Sciences | N/A |
| G-Deleted Rabies-eGFP | Janelia Viral Tools | N/A |
| G-Deleted Rabies-mCherry | Janelia Viral Tools | N/A |
| HSV-hEF1a-Rabies G | Gene Delivery Technology Core Massachusetts General Hospital | RN700 |
| HSV-hCMV-YTB | Gene Delivery Technology Core Massachusetts General Hospital | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Carbenoxolone | Sigma-Aldrich | C4790 |
| 18ß-glycyrrhetinic acid | Sigma-Aldrich | G10105 |
| Meclofenamic acid | Fisher Scientific | AAJ6048403 |
| Tetrodotoxin | Abcam | ab120054 |
| Substance P | Abcam | ab120170 |
| DAMGO | Abcam | ab120674 |
| Nifedipine | Tocris | 1075 |
| Bay K8644 | Tocris | 154410 |
| Veratridine | Abcam | ab120279 |
| ZD7288 | Abcam | ab120102 |
| Riluzole | Abcam | ab120272 |
| Mibefradil | Tocris | 219810 |
| Iberiotoxin | Alomone Labs | STI-400 |
| UCL1684 | Tocris | 13105 |
| QX314 chloride | Tocris | 2313 |
| NBQX | Abcam | ab120046 |
| CNQX | Abcam | ab120044 |
| D-APV | Alomone Labs | D-145 |
| Picrotoxin | Abcam | ab120315 |
| Strychnine | Sigma Aldrich | S0532 |
| Cholera Toxin Subunit B, Alexa Fluor™ 555 Conjugate | Life Technologies | C34776 |
| Experimental models: organisms/strains | ||
| Mouse: C57Bl/6J | The Jackson Laboratory | JAX: 000664 |
| Mouse: Snap25-GCaMP6s (Snap25tm3.1Hze) | The Jackson Laboratory | JAX: 025111 |
| Mouse: Slc17a6-Cre | The Jackson Laboratory | JAX: 016963 |
| Mouse: Gad2-Cre | The Jackson Laboratory | JAX: 010802 |
| Mouse: Slc32a1-Cre | The Jackson Laboratory | Jax: 028862 |
| Mouse: Slc6a5-Cre | Sherman et al. 2015 | N/A |
| Mouse: RIKEN-Slc6a5-Cre | RIKEN BRC | RBRC: 10109 |
| Mouse: Dbx1-Cre | Bielle et al. 2005 | N/A |
| Mouse: Foxp2-Cre | The Jackson Laboratory | JAX: 030541 |
| Mouse: Chat-Cre | The Jackson Laboratory | JAX: 006410 |
| Mouse: Egr2-Cre | The Jackson Laboratory | JAX: 025744 |
| Mouse: Neurod6-Cre | Schwab et al. 2000 | N/A |
| Mouse: Penk-Cre | The Jackson Laboratory | JAX: 025112 |
| Mouse: Parv-Cre | The Jackson Laboratory | JAX: 017320 |
| Mouse: Tac1-Cre | The Jackson Laboratory | JAX: 021877 |
| Mouse: Npy-Cre | The Jackson Laboratory | JAX: 027851 |
| Mouse: ROSA-LSL-G-TVA (Gt(ROSA)26Sortm1(CAG-RABVgp4,-TVA)Arenk) | The Jackson Laboratory | JAX: 024708 |
| Mouse: ROSA-LSL-GCaMP6s (Ai96) | The Jackson Laboratory | JAX: 028866 |
| Mouse: ROSA-LSL-GCaMP6f (Ai95) | The Jackson Laboratory | JAX: 028865 |
| Mouse: ROSA-LSL-EYFP | The Jackson Laboratory | JAX: 006148 |
| Mouse: Slc17a6-Flp0 | The Jackson Laboratory | JAX: 030212 |
| R26 LSL FSF ReaChR-mCitrine | The Jackson Laboratory | JAX: 024846 |
| RC::FLTG | The Jackson Laboratory | JAX: 026932 |
| Software and algorithms | ||
| Matlab | MathWorks | |
| Whistles | Holy and Guo, 2005 | |
| Custom Matlab code | Yackle Lab | DOI: 10.5281/zenodo.5765668 |
| Other | ||
| M.O.M.® (Mouse on Mouse) Blocking Reagent | Vector Laboratories | MKB-2213-1 |