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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Bioorg Med Chem Lett. 2022 Jan 19;60:128571. doi: 10.1016/j.bmcl.2022.128571

Figure 1.

Figure 1.

Examples of coumarin-based LC stabilizers and their associated activities. For stabilizer 1, the 3-and 7-position carbons are labeled. The EC50 is defined as the concentration of stabilizer that affords 50% maximal activity (i.e., proteinase K protection) for fluorescein-labeled WIL-FL (WIL-FL*, 5 to 20 nM dimer) using the protease-coupled fluorescence polarization assay. The T46L/F49Y variant replaces two residues in the stabilizer binding site to the residues most commonly found in AL LC sequences34–35. Note that stabilizers 2 and 3 were referred to as 26 and (R)-83, respectively, in our previous study14.