Fig. 5.

miR-1246 modulates PI3K/AKT signaling by targeting PIK3AP1. IGF-1, an activator of the signaling pathway of PI3K/AKT, was added at the same time of miR-1246 transfection. a Levels of protein for PIK3AP1, p-PI3K, PI3K, p-AKT, and AKT in TPC-1 and FTC-133 cells detected using Western blotting. b, c CCK-8 analysis of TPC-1 and FTC-133 proliferation. d Transwell analysis of the migration and invasion ability of these cells (magnification, 200×). e Apoptosis levels of these cells analyzed using FCM. *P < 0.05. PIK3AP1 phosphoinositide 3-kinase adapter protein 1, PI3K/AKT phosphoinositol 3-kinase/protein kinase B, IGF-1 insulin growth factor-1, CCK-8 cell counting kit-8, FCM Flow cytometry, NC negative control