Fig. 6.

Overexpression of miR-1246 inhibits the growth of tumors and metastasis in lung tissues. After transfection of TPC-1 and FTC-133 cells with miR-1246 agomir, bred for 6 weeks and then sacrificed, subcutaneous tumorigenesis and lung metastasis assays (caudal vein injection) were performed. a Image of the tumor 6 weeks after subcutaneous xenograft. b Calculation of tumor volume. c The expression of miR-1246 in tissues of the tumor was analyzed through RT-qPCR. d HE staining results of lung tissue (200×). e Calculation of tumor nodules in lung tissue. The groups are as follows: TPC-1-miR-1246 agomir, TPC-1-miR-1246 agomir NC, FTC-133-miR-1246 agomir, and FTC-133-miR-1246 agomir NC groups. *P < 0.05. NC negative control