Fig. 1.

MPP⁺ induced SH-SY5Y cell pyroptosis by inflammasome activation. SH-SY5Y cells were treated with MPP⁺ (0.25, 0.5,1 mM) for 24 h and frequency of pyroptotic cells measure by flow cytometry. Frequency of pyroptotic cells was quantified by calculation of the internalization of PI and caspase-1 activation in the cells (A). Summary data for the frequency pyroptotic SH-SY5Y cells in the indicated groups as in A. The frequency of pyroptotic cells was increased along with the concentration of MPP⁺ (B). Expression of inflammasome effector proteins Gsdmd-full length, Gsdmd-N, pro-IL1β, IL-1β, pro-caspase-1, caspase-1 p20 in SH-SY5Y cells from the indicated group was detected by western blot assay. MPP⁺ treatment significantly induced cleavage of Gsdmd, pro-IL-1β, and caspase-1 and had no impact on the protein levels of Gsdmd, pro-IL-1β, and caspase-1 pre-cleaved peptides (C). NLRP3/ASC interaction detected following immunoprecipitation with anti-ASC or control IgG antibody (D). Results represent mean ± SD of three individual experiments for each condition, performed in triplicate. *p<0.05, **p<0.01