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. 2021 Oct 14;16(3):842–855. doi: 10.1038/s41396-021-01132-4

Fig. 5. Early evolved filament system in Sulfurihydrogenibium spp. is encoded and transcribed from CRISPR-targeted plasmid.

Fig. 5

A Plasmid sequences from the Deep Hole and Liberty Cap are compared and RNA expression is reported as reads per kilobase per megabase (RPKM) for the Liberty Cap sequence. Hash marks in the beta-barrel porin bar indicate RNA expression of 3.6, beyond the limits of the y-axis. B Four CRISPR target sites are indicated along the plasmid sequence from the Deep Hole. C A conserved gene neighborhood in both plasmid sequences encodes a complete TFF system [OM outer membrane, IM inner membrane, IMP inner membrane platform]. D, E Scanning electron micrographs of biofilm matrices at (D) the Deep Hole and (E) Liberty Cap. F Bayesian phylogeny of deduced secretin protein sequences from Sulfurihydrogenibium plasmids detected in three regions of YNP. Posterior probabilities are indicated by circles at nodes. Asterisk indicates the clade containing secretin genes from the genomic (non-plasmid) DNA of Sulfurihydrogenibium.