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. 2021 Oct 9;16(3):812–821. doi: 10.1038/s41396-021-01130-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Meropenem MIC curves for PAO1, S. maltophilia K279a and S. maltophilia K279a ampR^FS. b Growth of PAO1 while in coculture with either K279a or K279a ampR^FS during meropenem treatment, orange line shows PAO1 monoculture control. c Assay to detect the inactivation of meropenem by K279a. The ability of PAO1 to grow in sterile filtered supernatant following 24 h incubation/growth in the presence of meropenem with no inoculum, K279a, K279a ampR^FS or PAO1. Line colours represent the inoculum of the initial round of growth from which the supernatant was sourced. a and c bold lines show mean and b the median of six biological replicates that are represented by narrow lines of the same colour. a and c the shaded areas show standard deviations (n = 6). b the horizontal dashed line shows the initial inoculum density of PAO1 (5 × 10⁵ CFU/ml).