Table 1.
The sequence and concentration of primer and probe sets used in PCR reactions.
| Primer/probe | Sequence (5′–3′) | Working conc. (µM) | Final conc. (µM)a | Size (bp) | Reference |
|---|---|---|---|---|---|
| RdRp—F | CCTCACTTGTTCTTGCTCGC | 20 | 1 | 205 | This study |
| RdRp—R | GCCGTGACAGCTTGACAAAT | 20 | 1 | ||
| RdRp—Probe | FAM-GTGAAATGGTCATGTGTGGC-BHQ1 | 5 | 0.2 | ||
| N–F | TGAAACTCAAGCCTTACCGC | 20 | 1 | 160 | This study |
| N—R | TATAGCCCATCTGCCTTGTG | 20 | 1 | ||
| N—Probe | HEX-ATCCATGAGCAGTGCTGAC-BHQ | 5 | 0.2 | ||
| RP—F | AGATTTGGACCTGCGAGCG | 20 | 1 | 92 | Universal |
| RP—R | GATAGCAACAACTGAATAGCCAAGGT | 20 | 1 | ||
| RP—Probe | ROX-TTCTGACCTGAAGGCTCTGCGCG-BHQ2 | 5 | 0.2 |
aFinal concentration represents the concentration of each oligonucleotide in reaction mixture (20 µL).