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. 2022 Feb 18;12:2788. doi: 10.1038/s41598-022-06545-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A–F) Light micrographs showing portions of capsule from sections subjected to picrosirius-red and analyzed under polarized illumination. At 7 days, thin birefringent collagen fibers (in red-orange) are dispersed in the capsules. At 60 days, the capsules exhibit thick bundles of birefringent collagen; note an accentuated birefringence in capsules of BIO (D) and CG (F) groups compared to the CAL group (E). Bars: 18 µm. (G) The graphic shows the values (expressed as mean ± standard deviation) of the amount of birefringent collagen (in percentage) in the capsules. In each period, the comparison among the groups is indicated by superscript letters; different letters = significant difference. The superscript numbers indicate the analysis of each group over time; different numbers = significant difference. Tukey’s test (p ≤ 0.05).