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. 2022 Feb 18;12:2847. doi: 10.1038/s41598-022-06678-7

Figure 1.

Figure 1

PAD2 is upregulated in RA-ILD fibroblasts. (A) Control (n = 4) and RA-ILD (n = 6) lungs were digested and subjected to total RNA isolation. mRNA levels of PAD2 were measured by RT-PCR. (B) Sorted mesenchymal (Mes) cells from control (n = 6) and RA-ILD (n = 6) lungs were subjected to total RNA isolation. mRNA levels of PAD2 were measured by RT-PCR as described in the Methods. (C) Primary human lung fibroblasts from control (n = 4) and RA-ILD (n = 4) lungs were isolated as described in the Methods. Data are mean ± SEM. P < 0.05; significant comparisons Student’s unpaired t test: *vs. control. Abbreviations: ILD = interstitial lung disease, PAD2 = peptidylarginine deminase 2, RA = rheumatoid arthritis, RT-PCR = real-time polymerase chain reaction.