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. 2022 Feb 18;12:2820. doi: 10.1038/s41598-022-06751-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proteinase activity, measured as relative fluorescence units (RFU) in the matrix extracts, normalized by weight of the tooth, showed no significant differences in matrix proteinase activity between Wdr72^+/+(WT), Wdr72^+/−(Het), and Wdr72^−/− (KO) ameloblasts. (A) Incisors containing all stages of enamel matrix formation, and (B) maturation stage enamel matrix from postnatal day 14 (P14) molar had similar activity at all time points. (C) The right panel shows the relative quantitative real-time PCR (qPCR) of micro-dissected maturation-stage ameloblasts from first molars. Relative expression levels of Klk4 and Wdr72 were analyzed by the ddCt method. The expression of each gene was calculated as a fold change compared to the control. The significance of differences was determined by a paired t-test. P < 0.05 was considered significantly different.