Table 2.
Macroscopic dissociation constants for 1:1 and 1:2 protein-metal complexes of UipAext proteins and selected metals.
| Metal | UipAext-ViU2A | UipAext-HG3 | UipAext-A9 | |||
|---|---|---|---|---|---|---|
| Prot-Me | Prot-Me2 | Prot-Me | Prot-Me2 | Prot-Me | Prot-Me2 | |
| Uranyl (UO22+) | (4.2 ± 1.8) × 10–8 | (5.0 ± 1.1) × 10–8 | (3.1 ± 2.5) × 10–8 | (3.7 ± 1.5) × 10–8 | (1.3 ± 0.6) × 10–9 | (3.6 ± 0.2) × 10–8 |
| Iron (Fe3+) | (3.3 ± 1.1) × 10–9 | (2.3 ± 0.1) × 10–8 | (2.9 ± 0.2) × 10–8 | (2.6 ± 0.4) × 10–5 | (3.2 ± 0.3) × 10–8 | (4.4 ± 0.1) × 10–5 |
| Calcium (Ca2+) | (1.1 ± 0.7) × 10–4 | (6.9 ± 0.7) × 10–4 | (1.2 ± 0.5) × 10–4 | (2.5 ± 0.2) × 10–3 | (2.9 ± 1.6) × 10–4 | – |
| Nickel (Ni2+) | (1.7 ± 0.3) × 10–5 | (2.2 ± 0.1) × 10–5 | (4.0 ± 0.9) × 10–5 | (2.8 ± 0.2) × 10–5 | (1.8 ± 0.8) × 10–4 | – |
| Zinc (Zn2+) | (8.9 ± 3.2) × 10–5 | (1.3 ± 0.1) × 10–4 | (3.3 ± 0.9) × 10–4 | (6.9 ± 0.4) × 10–5 | (5.5 ± 4.4) × 10–5 | – |
Values are given in M ± corresponds to the standard deviation, calculated from three independent experiments.