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. 2022 Feb 18;12:2828. doi: 10.1038/s41598-022-06892-3

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© The Author(s) 2022

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The Safranin O staining for evaluating the cartilage at × 100 magnification. (A–C) joint area. (D–F) cement line. Staining with red areas refer to articular cartilage; blue areas refer to calcified cartilage. Hyperchromatic granules within the calcified cartilage refer to bone marrow.