TABLE 5.

Detection of WN virus in human CSF specimens by NASBA, TaqMan, and RT-PCR assays^a

Sample no.	WN virus serologyb	NASBA-ECL assay result		TaqMan assay		RT-PCR result
		ECL units	Interpretationc	CT value	Interpretationd
1	POS	578,934	POS	35.7	POS	NEG
2	POS	65,745	POS	36.2	POS	NEG
3	POS	39	NEG	37	POS	NEG
4	POS	219,583	POS	38.3	EQUIV	NEG
5	POS	2,176	POS	39.3	EQUIV	NEG
6	POS	222,392	POS	45	NEG	NEG
7	POS	189,660	POS	39.67	EQUIV	NEG
8	POS	18,395	POS	35.2	POS	NEG
9	POS	35	NEG	45	NEG	NEG
10	POS	10	NEG	45	NEG	NEG
11–20	All NEG	All <50	All NEG	All 45	All NEG	All NEG

^aAbbreviations: POS, positive; NEG, negative; EQUIV, equivocal. 

^bWN virus serology positive is defined as positivity by IgM and PRNT assay. 

^cNASBA-ECL assay units >300 are interpreted as positive. 

^dC_T values <37 and Rn values greater than two times the average fluorescence for negative control samples is interpreted as a positive TaqMan assay result. For complete explanation of TaqMan assay: data interpretation, see Materials and Methods.