Table 1.
Composition and Process of making βC NLC, βC-emulsion, βC -Tween, βC-PBS.
| Components | βC NLC | βC emulsion | βC -Tween | βC -PBS |
|---|---|---|---|---|
| PS-PO (lipid phase ratio) | 5.5:45 w/w | 5.5:45 w/w | - | - |
| BC concentration | 250 μg/mL | 250 μg/mL | 250 μg/mL | 250 μg/mL |
| Tween 80 | 1:4.9 w/w (lipid: surfactant ratio) | 1:4.9 w/w (lipid: surfactant ratio) | 0.01% | - |
| Lipid + surfactant | 26% of final solution (w/w) | 26% of final solution (w/w) | - | - |
| PBS Water (pH = 7) | 76% PBS water (w/w) | 76% PBS water (w/w) | Added until 100 mL of mixture | Added until 100 mL of mixture |
| Process | Homogenized with ultra-turrax at 24,00 rpm, 30 min Sonicated at 20 kHz, 15 min |
Homogenized with magnetic stirrer at 600 rpm, 30 min | ||